Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets.
It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcR?) and natural killer receptors.
Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation.
This procoagulant function may contribute to arterial and venous thrombus formation.
The signaling pathway involves the FcR?, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2.
GPVI deficiency can result in bleeding disorders.
Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlImmunofluorescence: 20ug/ml Immunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Recommended Positive Control: A20 Cell Lysate
Recommended Control Peptide: G4906-01D
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide
Specificity：Recognizes human GPVI. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa near the carboxy terminus of the human GPVI.
