eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription.
eIF4E also influences the overall rate of translation.
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Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA.
Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F.
eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32000Western Blot: 0.25-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-saline, 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human 4E-T at ~140-150kD in 293 lysates (predicted size of ~108kD according to NP_062817.1). Species Crossreactivity: Mouse and rat.
