84-1113-68　［取扱停止］Apolipoprotein M (ApoM, G3a, HSPC336, MGC22400, NG20, NG20-like protein) 100ug　A2299-42E

特徴

• Apolipoprotein M (ApoM) is a member of the lipocalin protein family.
• It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins.
• It is positively related to leptin.
• It is mainly expressed in liver and kidney.
• ApoM is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.
• It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:8000Western Blot: 0.2ug/ml.
• Preliminary experiments gave an approx 50kD band in human kidney and liver lysates after antibody staining.
• Currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kD according to NP_061974.2.
• We do note that ApoM is a glycoprotein which makes a higher size band to be expected.
• The 50kD band was successfully blocked by incubation with the immunizing peptide
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.