Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns.
A sequence polymorphism has been identified in the 3'UTR of the third exon.
The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles.
Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.
Applications:Suitable for use in ELISA and Western BlotOther applications not tested.
Recommended Dilution:ELISA: 1:32000Western Blot: 0.01-0.03ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes APOA4 at ~45kD in Human Duodenum lysates (calculated MW of 45.4kD according to NP_000473.2).