This protein belongs to the aldehyde dehydrogenases family of proteins.
Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism.
Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.
Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme.
A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
This gene encodes a cytosolic isoform, which has a high affinity for aldehydes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.03-0.1ug/mlOptimal dilutions to be determined by the researcher.
Titer:1:16,000 (by peptide ELISA)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide
Specificity：Recognizes human ALDH1A1 at ~55kD in human liver lysates (calculated MW of 54.9kD according to NP_000680.2). This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Species Crossreactivity: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the C-terminus of human ALDH1A1
