The anterior gradient protein-2 (AGR2) is a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen.
It has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells.
Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells (1).
Specifically, AGR2 may serve as a useful molecular marker and/or potential therapeutic target for hormone-responsive breast tumors (2).
AGR2 expression promotes tumor growth in esophageal adenocarcinoma cells as well.
Expression was detected in proliferating and differentiated intestinal cells of secretory lineage, suggesting that AGR2 may be important for the growth and development of the intestine as well as esophageal adenocarcinomas (3).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.01μg/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.5 mg/ml tris-saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human AGR2 at ~17-18kD in Human Colon, Duodenum and Lung lysates (calculated MW of 20.0kD according to NP_006399.1). Species Crossreactivity: Canine and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide corresponding to human AGR2.
