The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents.
Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas.
In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4).
The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1.
It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes Adenovirus-9 E4 Orf1.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Synthetic peptide corresponding to 16aa from near the carboxy terminus of the Ad-9 E4 Orf1.
