84-1113-03　Syndecan-1 (CD138, SDC, SYND1, Syndecan) 100ul　S9111-30B

特徴

• Syndecan-1 is a transmembrane (Type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family.
• The syndecans mediate cell binding, cell signaling, and cytoskeletal organization.
• Bearing properties of both heparan sulfate and chondroitin sulfate, Syndecan-1 functions as a cell surface proteoglycan membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins.
• Altered Syndecan-1 expression has been detected in several different tumor types.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 using mouse liver lysate.
• Proteins were visualized using a IgG (HRP) Dk x Rb and chemiluminescence detection.
• A dimer of Syndecan-1 was identified at ~77kD while monomeric Syndecan-1 is visible at ~32kD.
• The mature form, with heparan and chondroitin sulfate addtions, can sometimes be seen between 500-1000kD but may need to be solubilized from whole tissues by urea extraction.
• Triton X-100 or other mild detergents are enough to release the immature precursor forms
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-46E: IgG, H&L (HRP) Pab Dk x RbI1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab Dk x RbI1903-89G5: IgG, H&L, X-Adsorbed (HRP) Pab Dk x RbI1904-45P6: IgG, H&L (HRP) Pab Dk x Rb
• Positive Control:Mouse liver lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.