Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus.
Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7.
Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.
Following stimulation by TGF-beta, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-beta Receptor I.
Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 was used to detect Smad3 in UV-treated AKR-2B cell lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes phosphorylated Smad3 (Ser423 and Ser425). Species Crossreactivity: mouse and mink.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated, synthetic phosphopeptide corresponding to residues surrounding Ser423/425 of human Smad3. Cellular Localization: Cytoplasm. Species sequence homology: human and rat.
