Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c.
Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression.
Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor.
Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 detected RSK1 on 10ug of NIH/3T3 lysates
Immunoprecipitation: 4ug was used per 1500ug of crude protein sampleOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as liquid in TBS, 0.01% Sodium Azide, 50% glycerol.
Specificity：Recognizes human p90 RSK1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the CT sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
