Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation.
Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho.
Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers.
Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases.
The latter pathway is activated by the PAK kinases, which are direct targets of Rac.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.15M sodium chloride, pH 7.6, 0.09% sodium azide.
Specificity：Recognizes human Rac1. Does not cross-react with Cdc42. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa130-141 of human Rac1 (KEKKLTPITYPQ-C). Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side.
