Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC.
MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth.
Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin.
Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 detected phosphorylated MARCKS on 10ug of EGF treated A431 lysates
Immunohistochemistry (paraffin): 1:100 Optimal dilutions to be determined by the researcher.
Positive Control:A431 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat MARCKS when phosphorylated at Ser152/156. Species Crossreactivity: mouse and human.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
