HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains.
It is known to binds AU-rich elements if mRNA.
It regulates gene expression by destabilizing their mRNA.
It is known to be upregulated in certain cancers such as breast and gastric cancers.
Some of its targets include cFos, IL-3, and BRCA1 mRNAs.
HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types.
It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
*Handling Recommendations: To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes.
Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10uL of residual ammonium sulfate solution will not affect the re-suspension of the antibody.
Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Re-suspend the antibody pellet in 100ul either standard PBS or TBS (pH 7.3-7.5).
DO NOT VORTEX.
Mix by gentle stirring with a wide pipet tip or gentle finger-tapping.
Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use.
Small particles of precipitated antibody that fail to re-suspend are normal.
Vials are overfilled to compensate for any losses.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in 50% saturated ammonium sulfate* and PBS. No preservatives added.
Specificity：Recognizes human HuR. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminus of human HuR.
