84-1112-76　G-Protein Gi alpha1/alpha2 (Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1/Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2) 100ug　G8599-05A

特徴

• GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors.
• The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation.
• Gi belongs to the pertussis toxin sensitive G-proteins.
• Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:100.
• Requires HIER with citrate buffer, pH 6.0Optimal dilutions to be determined by the researcher.
• Positive Control:Western Blot: L6 Cell LysateImmunohistochemistry: Rat cerebellum
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.