GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways.
Two isoforms exist, GSK3α and GSK3β.
The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it.
Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3.
Glycogen Synthase is then rapidly dephosphorylated and activated.
Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B.
Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease.
Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Immunohistochemistry (paraffin): 1ug/ml Immunoprecipitation: 12.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:C2C12 Cell Lysate, Hela cells, rat or mouse brain
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes rat GSK3 beta. Species Crossreactivity: mouse, human and bovine.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa335-349 from rat GSK3 beta, conjugated to KLH. This sequence is 100% identical to human GSK3 beta.
