Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system.
GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals.
The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2.
The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene.
Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region.
Alternative splicing of this gene generates 4 transcript variants.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detects GABA B R1 on 10ug of rat brain lysate
Immunohistochemistry: 1:3000 in free floating mouse brain tissue
Optimal dilutions to be determined by the researcher.
Positive Control:Rat brain lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide.
Specificity：Recognizes GABA B Receptor R1 (GABA B R1). Species Crossreactivity: rat, mouse and human.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant peptide (aa972-991, PSEPPDRLSCDGSRVHLLYK) cocorresponding to GABA Receptor B R1. Epitope: C-Terminus interaction w/ ATF4GP.
