84-1112-72　［取扱停止］G3BP-1 (GTPase activating protein (SH3 domain) binding protein 1, ATP-dependent DNA helicase VIII, G3BP, G3BP-1, GAP SH3 domain-binding protein 1, HDH-VIII, MGC111040, Ras GTPase-activating protein-binding protein 1) 100ul　G0875-01A

特徴

• G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs).
• It was first discovered by its binding to RasGAP.
• G3BPs have been shown to be involved in a number of mitogenic signaling pathways.
• G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007).
• Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007).
• Furthermore, the G3BPs have two traditional RNA binding motifs.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 detects G3BP1 in HeLa, 293T and S10 cell lysatesOptimal dilutions to be determined by the researcher.
• Positive Control:HeLa cell lysates.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.