eIF4F is a translation initiation factor containing three subunits: eIF4A, eIF4E, and eIF4G.
This complex is a key component in promoting ribosomal binding to the mRNA.
eIF4G interacts with both eIF4A and eIF4E though its carboxy and amino terminal domains, respectively.
eIF4E binds to the cap structure of the mRNA, while the ATP-dependent RNA helicase eIF4A unwinds the secondary structure of the 5’ UTR.
eIF4G plays a critical role in coordinating the assembly of the eukaryotic initiation factors.
It acts as a bridge to bind the cap-bound eIF4E, eIF4AI&II, the eIF3 complex, PABP1, and the kinase Mnk1 (Lejeune, F., et al., 2004) in a large eukaryotic initiation factor complex
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000 detects eIF4G in HeLa cytoplasmic extract and lysates from 293T and S10 cells
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cytoplasmic extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide
Specificity：Recognizes human eIF4G at its N-terminal region. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to N-terminal region of human eIF4G
