84-1112-57　Activating enhancer-binding protein 2-beta (AP-2 beta, TFAP2B) 100ug　T8195-52E3

特徴

• AP-2 beta (Activating enhancer-binding protein 2-beta; also TFAP2B) is a 52kD member of the AP-2 family of transcription factors.
• It is primarily a fetal-expressed protein, being found in renal and skin epithelial cells, as well as neuroblasts plus cells of the facial mesenchyme.
• In adults, AP-2 beta is expressed in renal epithelium.
• Human AP-2 beta is 460aa in length.
• It contains a transactivation domain (aa41-131), a DNA-binding motif (aa132-298) and a C-terminal dimerization region (aa299-439).
• SUMOylation occurs on Lys21, adding 15kD of MW in SDS-PAGE.
• AP-2 beta both homodimerizes, and heterodimerizes with AP-2 alpha and -2 gamma.
• Dimers appear important for facial and limb development.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml using lysates of SH-SY5Y human neuroblastoma cell lineOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.