TCF7 (Transcription factor 7; also T cell factor 1/TCF1) is a 25-50kD member of the lymphoid enhancer binding factor family of proteins with 16 isoforms.
It is expressed in thymocytes and mature T cells, and serves multiple purposes.
In resting cells, TCF family members are transcriptional repressors, and are 25-32kD in size.
Following activation, large TCF7 isoforms predominate (42-50kD), and serve a transcriptional activator function.
Human TCF7 is 384aa in length.
This is likely an activating isoform that contains a beta-catenin binding domain (aa1-59), a DNA-binding HMG-box (aa269-337), and an NLS (aa344-348).
The use of an alternate start site at Met116 seems to characterize repressor isoforms.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human TCF7/TCF1 at~60, 40, and 38kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human TCF7/TCF1 aa116-233; P36402 (E. coli).
