TRF-2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60kD, ubiquitously expressed nuclear protein that participates in telomere homeostasis.
TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway.
It also collaborates with TRF-1 to promote normal telomere length.
TRF2 has been found up regulated in several human cancers.
Human TRF-2 is 500 aa in length.
It contains an N-terminal Arg-rich region (aa13-30), a dimerization domain (aa46-112), an NLS (aa329-333), and a DNA binding HTH myb-type domain (aa442-499).
There is one potential alternate start site 42aa upstream of the standard start site, and one splice form that shows a 13aa substitution for aa239-500.
Over aa78-238, human TRF-2 shares 96% aa identity with mouse TRF-2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human TRF2 at ~60kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human TRF2 aa78-238; Q15554 (E. coli).
