LKB1 (also STK11 and JBS) is a 55kD member of the CAMK Ser/Thr protein kinase family.
It is ubiquitously expressed, and considered to be a tumor suppressor.
LKB1 is both nuclear and cytoplasmic, and appears to contribute to cell polarization, G1 cell cycle arrest and Wnt signaling.
It is known to complex with STRAD and CAB39/MO25, and phosphorylate PTEN plus p53.
Human LKB1 is 433 amino acids (aa) in length.
It contains a protein kinase domain (aa 49-309) and a prenylation motif (CysLysGlnGln) over aa 430-433.
Phosphorylation on Ser428 promotes the ability of LKB1 to suppress G361 cell growth.
There are two potential isoforms of LKB1.
One shows a nine aa insertion after Tyr126, while another shows a 34 aa substitution for aa 371-433.
Over aa 242-433, human and mouse LKB1 are identical in aa sequence.
Applications:Suitable for Western Blot.
Other applications not tested.
Storage:Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C to -70°C.
Upon reconstitution, the antibody can be stored at 2°-8°C for 1 month without detectable loss of activity.
Reconstituted antibody can also be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for six months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose.
Specificity：Recognizes endogenous human LKB1 when phosphorylated at Ser248 at ~62kD using Western blot.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthertic phosphopeptide of human LKB1 phosphorylated at the Ser428 site.
