SNF1LK2 (Sucrose non-fermenting 1 like kinase 2) also known as SIK2 (Salt-inducible kinase 2) is a cytoplasmic 125kD member of the CAMK Ser/Thr protein kinase family, AMPK subfamily of enzymes.It is expressed in preadipocytes/adipocytes, with levels strongly induced during early differentiation.SNF1LK2 regulates the early phase of insulin signaling by phosphorylating IRS-1 on Ser794.Human SNF1LK2 is 926 amino acids (aa) in length and contains a protein kinase domain (aa 20-271) with an ATP binding site (Lys49) and a catalytic loop (aa 140-149), followed by a phosphorylation activation site at Thr175, a UBA (ubiquitin-associated) domain (aa 295-335) and another PKA-dependent phosphorylation site at Ser587.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.
Specificity：Detects endogenous human SNF1LK2 at 125kD using Western blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived recombinant human SNF1LK2 (rhSNF1LK2; aa 663-818; Accession# Q9H0K1).
