Semaphorin 4B (Sema4B; also SemaC) is a 95-100kD, class IV member of the Semaphorin family of proteins.
It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses.
Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length.
It contains a 674 aa extracellular region (aa 39‑832) that is characterized by one Sema domain (aa 65‑502), a PSI region (aa 520‑574), and an Ig-like C2-type domain (aa 599‑658).
There is one potential soluble splice variant that shows a 29 aa substitution for aa 707‑832.
Over aa 39‑712, human Sema4B shares 86% aa identity with mouse Sema4B.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using human brainFlow Cytometry: 0.25ug/10e6 cells using THP-1 human acute monocytic leukemia cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Semaphorin 4B extracellular domain. Species Crossreactivity: ~15% crossreactivity with recombinant mouse Semaphorin 4B is observed and less than 1% crossreactivity with recombinant human (rh) Semaphorin 3A, 4A, and 7A is observed.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu39-Glu712 from human Semaphorin 4B isoform 1, expressed in NS0 cells.
