SCAMP3 (Secretory carrier membrane protein 3) is a 38-40kD member of the SCAMP family of proteins.
It is expressed in heart and skeletal muscle, plus fat, and functions in post-Golgi recycling and EGFR internalization pathways.
Human SCAMP3 is 347aa in length.
It contains a cytoplasmic N-terminus (aa1-170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa’s, and a potential SH3-binding motif.
This is followed by four consecutive transmembrane domains (aa171-297) plus a C-terminal cytoplasmic region (aa298-347).
It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR.
There are three potential splice forms.
One shows a deletion of aa 23-48, while two others show a one and 23 aa substitution for aa131-144 and 131-260, respectively.
Over aa1-170, human and mouse SCAMP3 are 91% aa identical.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human/mouse SCAMP3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant human SCAMP3, aa1- 170; O14828 (E. coli).
