c Kit, also known as CD117 and stem cell factor receptor, is a 145kD transmembrane tyrosine kinase encoded by the c Kit proto-oncogene.
c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion.
Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation.
c Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer.
Phosphorylation at tyrosine 936 of c Kit allows binding of Grb2 and activation of the Ras, Raf, ERK1&2 signaling pathway.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.4ug/mlWestern Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with BSA. Reconstitute with PBS.
Specificity：Recognizes canine SCF.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca
Immunogen：Recombinant protein corresponding to human Canine Stem Cell Factor, Lys26-Ala190, expressed in E. coli.
