Renin R (Renin Receptor; also ATPase H + -transporting lysosomal accessory/interacting protein 2, M8.9 and ELDF10) is a 39-45kD protein that belongs to no known family.
It is expressed on the surface of macrophages, vascular smooth muscle cells, renal mesangial cells and T cells.
Renin R has at least two functions.
First, it binds both renin and prorenin, promoting the conversion of angiotensinogen to angiotensin I.
Second, its ligation induces PAI-1 synthesis.
Mature human Renin R is a type I transmembrane protein 334 amino acids (aa) in length.
It contains a 286 aa extracellular region (aa 17-302) and a 27 aa cytoplasmic tail.
It is believed to form homodimers.
Potential isoforms exist.
One termed M8.9 shows a deletion of aa 1-252 and constitutes a component of the vacuolar proton ATPase.
Two others show an alternate start site at Met77, and a deletion of aa 101-132.
Over aa 17-302, the human Renin R shares 95% aa identity with the mouse Renin R.
Applications:Suitable for use ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Renin R.
The detection limit for rhRenin R is approximately 0.5ng/wellWestern Blot: 1ug/ml.
Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.
Specificity：Recognizes human Renin R extracellular domain.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived, recombinant human Renin R extracellular domain (rhRenin R; aa 17-302; Accession # O75787).
