15-PGDH (15-hydroxyprostaglandin dehydrogenase NAD-linked; also HPGD) is a 28-29kD member of the short-chain non-metalloenzyme family of dehydrogenases.
It is widely expressed, and antagonizes COX-2 by catalyzing the oxidation of 15-OH prostaglandin to metabolically inactive 15-keto metabolites.
Human 15-PGDH is 266aa in length.
A Tyr at position #151 serves as a proton acceptor, and a Ser at #138 binds substrate.
15-PGDH functions as a nondisulfide-linked homodimer.
There is one alternate splice form that shows a 12aa substitution for aa167-266.
Full-length human 15-PGDH shares 88% aa identity with mouse 15-PGDH.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human 15-PGDH.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human 15-hydroxyprostaglandin dehydrogenase aa1-266; P15428 (E. coli).
