84-1112-32　PIAS2 (Protein inhibitor of activated STAT 2) 100ug　P4085-11

特徴

• PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-β) is a 45-70kD member of the PIAS family of molecules.
• In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis.
• However, it is widely expressed in cultured cell lines.
• PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK-1, promoting its activation via HDAC2 dissociation and desumoylation.
• Human PIAS2 is 621 amino acids (aa) in length.
• It contains a SAP domain (aa11-45), a zinc-finger region (aa331-408), a SUMO-binding motif (aa467-472) and an NLS (aa484-492).
• There are multiple splice variants.
• PIAS2x-β/ARIP3 shows a 22 aa substitution for aa 551-621, while PIAS-NY shows a 12 aa substitution for aa 1-8, followed by a truncation after Gly401.
• Two others show a four aa substitution for aa 504-621, and an alternate start site at Met10.
• Over aa 1-550, human PIAS2 shares 98% aa identity with mouse PIAS2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml detects PIAS2 in lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell lineOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.