Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22kD, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins.
The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54-63, and a peroxisome targeting motif at aa 208-210.
There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor.
Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes.
It is known to reduce H 2O2 and alkyl hydroperoxides.
Two potential splice forms are reported.
One is 17kD in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83-102.
Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.1ml of PBS is used, the antibody concentration will be 1mg/ml.
Specificity：Detects endogenous human, mouse and rat Peroxiredoxin 5 at ~22kD by Western blot.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified, E. coli-derived, recombinant mouse Peroxiredoxin 5 (rmPrx-5; aa 1-210; Accession# P99029)
