84-1112-28　PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin, Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-containing protein 2 Pigment epithelium-derived factor, Transport-secretion protein 2, TTS2, TTS2.2, TTS-2.2) 100ug　P4350-15

特徴

• PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55kD member of the PNPLA family of proteins.
• It is expressed in adipocytes, hepatocytes, skeletal muscle and pigment epithelium.
• PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids.
• Mouse PEDF R is described as being a 486aa type II transmembrane (TM) protein.
• Its TM segment is stated to span aa9-29.
• Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295.
• It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif.
• There are multiple splice forms.
• Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa163-196 and 253-308, respectively.
• Over aa 62-253, mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively.
• Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1.0ug/mlImmunohistochemistry: 5-15ug/ml detects PNPLA2 in cells and tissuesDirect ELISA: 0.5-1.0ug/ml.
• The detection limit for recombinant mouse and recombinant human PNPLA2 is ~1ng/wellOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.