84-1112-27　PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes) 100ug　P3139-86

特徴

• PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis.
• It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes.
• Human PEA-15 is 130aa in length.
• It contains a death effector domain (aa3-81), plus two regulatory Ser phosphorylation sites.
• In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116.
• Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation.
• Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis.
• And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation.
• There is one potential alternate start site 21aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36-58.
• Full-length human and mouse PEA-15 show 99% aa identity.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile 40-50% glycerol, PBS..
• Aliquot and store at -20°C.
• Reconstituted product is stable for 6 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.