84-1112-25　Parathyroid hormone receptor 1 (PTH/PTHr receptor, PTH1 R) 100ug　P3109-51C

特徴

• PTH1 R (Parathyroid hormone receptor 1; also PTH/PTHr receptor) is a predicted 66-68kD member of the G-protein coupled receptor family 2.
• It is expressed on a variety of cell types, including hepatocytes, renal epithelium, smooth muscle cells, and osteoblasts plus chondrocytes.
• PTH1 R is a receptor for both PTH and PTHrP, and PTH binding promotes Ca ++ -release from bone, mediated by osteoclast formation.
• Mature human PTH1 R is a 7-transmembrane glycoprotein 567 amino acids (aa) in length.
• It contains a long, ligand-binding N-terminal extracellular region (aa 27-188) and a 130 aa cytoplasmic C-terminal domain.
• Single aa changes such as Gly121Glu, Ala122Thr and Arg255His can impair PTH1 R signaling.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/ml HUVEC human umbilical vein endothelial cells Immunohistochemistry: 5-15ug/ml.
• Immersion fixed paraffin-embedded sections of human kidney sibjectde to Antigen Retreival Reagent-Basic Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months after receipt at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.