Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD, 2538aa member of the notch family of type I TM glycoproteins.
It is associated with gene activation in both embryo and adult.
Applications:Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using bEnd.3 mouse endothelioma cell lineImmunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of mouse neural tube (E13.5)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Notch-1. In Direct ELISA and Western Blot, there is ~10% crossreactivity with rhNotch-1 and rrNotch-1. There is <1% crossreactivity with rmNotch-2 and rmNotch-3.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa19-526 from mouse Notch-1 expressed in CHO cells (AAM28905).
