NFATC1 (Nuclear factor of activated T cells c1; also NFAT2a) is a 120kD member of the NFAT family of transcription factors.
NFATC1 is found in T cells and mast cells, and regulates cytokine transcription.
NFATC1 is normally cytoplasmic and phosphorylated at Ser172.
Upon a rise in intracellular Ca +2 , dephosphorylation occurs via calcineurin, and NFATC1 enters the nucleus.
Human NFATC1 is 943aa in length.
It contains a calcineurin-binding site (aa118-123), two transactivation domains (aa126-218 and 703-943), two NLSs (aa265-267 and 682-684), an NES (aa310-321) and an RHD that binds DNA (aa410-592).
There are multiple isoforms.
Individually or in common, there is an alternate start site at Met473, a deletion of aa826-943, a 29aa substitution for aa1-42, and a 19aa substitution for aa 698-943.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml using Jurkat human acute T cell leukemia cell line, Ramos human Burkitt’s lymphoma cell line and Raji human Burkitt’s lymphoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Nuclear factor of activated T cells c1 at ~110-120kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala48-Ser406 from human Nuclear factor of activated T cells c1 expressed in E. coli. UniProt Accession: O95644
