Nepmucin (a not expressed in Peyer’s Patch mucin; also CLM-9) is an 80-95kD member of the CD300L family of proteins.
It is expressed on endothelium and serves multiple functions.
It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM.
Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein.
Its extracellular region (aa 19-288) contains one V-type Ig-like domain (aa 19-124) plus an O-glycosylated mucin region (aa 136-276).
There are three isoform variants.
One is 60-75kD in size and shows an Ala substitution for aa 126-170, a second is 46-50kD in size and shows an Arg substitution for aa 170-254, while a third is 36kD in size and shows a SerTyr substitution for aa 134-256.
Over aa 1-284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA. Reconstitute with 250ul sterile PBS
Specificity：Recognizes mouse Nepmucin. Shows less than 1% crossreactivity with recombinant human Nepmucin
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu19-Ile284 from mouse Nepmucin expressed in NS0 cells
