84-1112-17　Neuropilin-1 (NRP1, BDCA4, CD304, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein) 100Tests　N1276-91C

特徴

• Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells.
• Neuropilin-1 binds members of the class III secreted Semaphorin subfamily as well as some isoforms of VEGF family proteins.
• The aa sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 cells.
• Validated on bEND.3 cells and PC-12 cells
• Optimal dilutions to be determined by the researcher.
• Flow Cytometry Protocol:1.
• Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT.
• Do not wash excess blocking IgG from this reaction.
• 2.
• After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT
• 3.
• Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog #F4568-10).
• Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer
• 4.
• The cells were resuspended in Flow Cytometry Staining Buffer for analysis by Flow Cytometry.
• As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Carboxyfluorescein conjugates are sensitive to light.