The Netrin-G1 ligand (NGL-1) promotes the outgrowth of thalamocortical axons.
Human netrin G1 ligand is a transmembrane protein containing leucine-rich repeat (LRR) and immunoglobulin (Ig) domains that specifically interacts with netrin-G1 through its LRR region.
Postsynaptic netrin-G ligand (NGL) CAMs associate with netrin-G CAMs in an isoform-specific manner and, through their cytosolic tail, with the abundant postsynaptic scaffold postsynaptic density-95 (PSD-95).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Westerm Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human NGL-1.
The detection limit for rhNGL-1 is approximately 2ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS, 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base,150mM NaCl), 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
Specificity：Recognizes human NGL-1, extracellular domain.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, NS0-derived, recombinant human NGL-1 extracellular domain (rhNGL-1)
