84-1112-12　MCM2 (Minichromosome Maintenance Protein 2) 100ug　M2749-30Z

特徴

• MCM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins.
• Although its predicted MW is 102kD, it runs anomalously at 125kD in SDS-PAGE.
• MCM2 forms a large, 450-600kD prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis.
• Human MCM2 is 904 amino acids (aa) in length (Accession # P49736).
• It contains one C4-type zinc finger region (aa329-355) and a P-loop NTPase region (aa485-659) and at least 12 Ser/Thr phosphorylation sites in the N-terminal one-third of the molecule.
• MCM2 has several potential isoforms.
• There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109-157, a deletion of aa 31-126, a Gly substitution for aa 113-132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa1-137.
• Over aa143-296, human MCM2 shares 99% aa identity with mouse MCM2.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml detects MCM2 in lysates of Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line
• Immunohistochemistry: 5-15ug/ml detects MCM2 in immersion fixed paraffin-embedded sections of human colon array Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.