STXBP1 (Syntaxin-binding protein 1; also p67, Munc18-1, N-Sec1 and Unc18A) is a 67kD member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins.
It is expressed in a variety of cells, presumably in those that exhibit granule exocytosis, and likely participates in (synaptic) granule release.
Ligands for STXBP1 include STX1A, 2 and 3, and it is possible that STXBP1 both suppresses STX activity, and serves as an anchor for STX-mediated vesicle fusion.
Human STXBP1 is 594aa in length.
It contains one SEC1 domain (aa41-575).
Phosphorylation at Ser313 may stimulate granule release.
There is one splice variant that shows a 28aa substitution for aa576-594.
This is restricted to neural tissue.
Over aa 80-226, human and mouse STXBP1 are absolutely identical in amino acid sequence.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1.0ug/mlWestern Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human, mouse, rat Syntaxin-BP1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant human Syntaxin-BP1 aa80-226; P61764 (E. coli).
