84-1112-08　Sphingomyelin phosphodiesterase 1 (Acid sphingomyelinase, ASM, aSMase) 100ug　L9189-95B

特徴

• SMPD1 (Sphingomyelin phosphodiesterase 1; also ASM) is a 70-85kD member of the acid sphingomyelinase family of enzymes.
• It is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide through the removal of phosphorylcholine.
• This generates second messenger components that participate in signal transduction.
• Mature human SMPD1 is 583aa in length.
• It contains one saposin B-type domain (aa85-169) that likely interacts with sphingomyelin, and a catalytic region (aa267-363).
• There are multiple isoforms.
• One is 57kD in size and results from proteolytic cleavage between Pro179-Pro197.
• Three others show aa substitutions; a 56 aa substitution for aa1-361, a two aa substitution for aa 363-629, and a 12aa substitution for aa363-418.
• Over aa62-628, human SMPD1 is 86% aa identical to mouse SMPD1.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1.0ug/ml.
• The detection limit for rhSMPD1 is ~0.5ng/well
• Western Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.