IL-10 Rβ (Interleukin 10 receptor beta; also IL-10R2, CD210b and CRF2-4) is an 80-85kD member of the type II cytokine receptor family of proteins.
It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human).
Mature mouse IL-10 Rβ is a type I transmembrane protein that is 330 amino acids (aa) in length.
It contains a 201 aa extracellular domain (aa 20-220) that possesses two fibronectin type III domains (aa 22-107 and 111-208).
There is one alternate start site at Met20.
Over aa 21-220, mouse IL-10 Rβ shares 87% and 74% aa identity with rat and human IL-10 Rβ, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
The detection limit for rmIL-10 Rβ is approximately 1ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Suppled as lyophilized from a 0.2um sterile-filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
Specificity：Recognizes mouse IL-10 Rβ. Shows approximately 20% cross-reactivity with rhIL-10 Rβ and less than 1% cross-reactivity with rhIL-10 R and rmIL-10 R.
