GABA A (γ-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels.
GABA binding to A-type receptors induces anion-selective ion channel opening.
These receptors are the principal fast inhibitory neuro- transmitter receptors in the CNS.
GABA A receptors are heteropentamer combinations of seven subunit types; alpha, beta, γ, δ, ε, θ, and π.
Three subunits, alpha, beta, and γ, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta and γ subunits.
The rat γ2 isoform is a 48kD, 436aa, 4 transmembrane protein with two terminal extracellular regions.
The ligand-binding region is in the N-terminus aa30-233.
The γ 2 subunit is part of the most common GABAA receptor combination in the mammalian brain (alpha 1 beta 2γ2).
GABA binds at alpha-beta interfaces, while benzodiazepine binds to alpha-γ interfaces.
There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa376-383.
PKC phosphorylates the long form at S381, while both the short and long forms are phos- phorylated at S365.
Phosphorylation blocks receptor activity.
The γ 2 subunits are also palmitoylated at multiple sites on cysteines that lay between aa415-461, facilitating membrane trafficking.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Sufficient for 10 mini-blots
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes rat GABA-A-R gamma 2 at ~44-47kD. Species Cross Reactivity: bovine, human, and mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Fusion protein from the cytosolic loop of the rat GABAA R γ2 sub-unit, C-Terminus.
