GABA A (γ-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels.
GABA binding to A-type receptors induces an- ion-selective ion channel opening.
These receptors are the principal fast inhibitory neuro- transmitter receptors in the CNS.
GABA A receptors are heteropentamer combinations of seven subunit types; alpha, beta, γ, δ, ε, θ, and π.
Three subunits, alpha, beta, and γ, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta and γ subunits.
The rat alpha 4 isoform is a 67kD, 517aa, 4 transmembrane protein with two terminal extracellular regions.
The ligand-binding re- gion is in the N-terminus aa14-221.
The alpha 4 subunit is unusual in that it does not seem to preferentially form channel complexes with the γ subunit.
In hippocampal neurons, alpha 4 subunits apparently form GABA receptors that do not cluster, but exist in diffuse net- works in nonsynaptic membranes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Sufficient for 10 mini-blots
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Specific for the ~64kD α 4 subunit of the GABAA Receptor in Western blots of rat brain lysates. Also recognizes the α 4 subunit of the GABAA Receptor in bovine, human and mouse brain lysates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to GABA-A-R alpha 4, N-Terminus conjugated to (KLH).
