84-1111-92　［取扱停止］GABA Receptor A, alpha 1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA) [KO Validated] 100ul　G1015-09D

特徴

• GABA A (γ-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels.
• GABA binding to A-type receptors induces anion-selective ion channel opening.
• These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS.
• GABA A receptors are heteropentamer combinations of seven subunit types; aplha, beta, γ, δ, ε, θ, and π.
• Three subunits, aplha, beta, and γ, have at least three separate gene products in mammals, and typical GABA A receptors have some combination of alpha, beta, and γ subunits.
• The rat alpha 1 isoform is a 50-52kD, 428aa, 4 transmembrane protein with two terminal extracellular regions.
• The ligand-binding region is in the N-terminus aa15-222.
• As with many receptors, phosphorylation is used as a regulatory mechanism.
• CaM kinase II is known to phosphorylate the aplha 1 subunit and regulate benzodiazepine binding.
• Alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution.
• In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000.
• Sufficient for 10 mini-blots
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.