Folate receptor 1 (FOLR1; also folate receptor alpha, folate binding protein 1, and MOv18) is a 37-42kD member of the folate receptor family.
FOLR1 is expressed in reticulocytes and placenta, plus renal, mammary and thymic epithelium, where it mediates the transport of 5-methyltetrahydrofolate into the cell.
The human FOLR1 preproprecursor is 257aa in length.
It codes for a GPI-linked glycoprotein that is 210 aa in size (aa25-234).
There are two forms of FOLR1.
One is GPI-linked, while the other is a soluble MMP cleavage product (aa25-226).
There is one potential alternate splice form that shows a five aa substitution for aa 121-205.
Over aa 25-233, human FOLR1 shares 83% aa identity with mouse FOLR1.
Applications:Suitable for use in Flow Cytometry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 25ug/10e6 cells using MCF-1 cellsWestern Blot: 1ug/ml using HeLa cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human FOLR1. Shows <15% crossreactivity with recombinant mouse FOLR1 and recombinant human FOLR3 in direct ELISAs.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells.
