ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70kD member of the NR3 subfamily, nuclear hormone receptor family of proteins.
It is widely expressed, and serves as a strong activator of estrogen-responsive genes.
ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins.
Following beta-estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators.
Human ER alpha is 595aa in length, and contains a DNA binding region (aa185-250), three NLSs (aa256-260; 266-271; 299-303), a steroid-binding site (aa351- 543), a dimerization motif (aa497-518), and an O-GlcNAc attachment around Thr575.
Major phosphorylation sites exist at Tyr537, Ser167 and Ser118.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS..
Aliquot and store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS) 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity：Recognizes human ER alpha at ~66kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Estrogen receptor alpha aa 1-116; P03372 (E.coli).
