DOPA decarboxylase (Aromatic-L-amino acid decarboxylase; also DDC) is a 54kD member of the alpha-family of pyroxidal phosphate enzymes.
Functional DDC is a homodimeric, pyridoxal phosphate-dependent enzyme that calatyzes the decarboxylation of DOPA to dopamine, L-tryptophan to tryptamine, and 5-hydroxytryptophan to serotonin.
Both dopamine and serotonin are important neurotransmitters.
It also demonstrates deaminase activity, and is regulated by O 2 availability.
Bovine DDC is 487aa in length and contains an N-terminal decarboxylase domain (aa35-414) and a C-terminal pyroxidal binding site (L303).
Bovine DDC is 88% and 86% aa identical to human and rat DDC, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Sufficient for 10 mini-blots
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：sheep
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes bovine DOPA Decarboxylase at ~55kD. Species Cross Reactivity: canine, guinea pig, human, rabbit, rat, and sheep.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Gp Hu Rb Rt Sh
Immunogen：SDS denatured recombinant bovine DOPA expressed in E. coli and purified from inclusion bodies.
