Dopamine beta-hydroxylase (DbetaH; also dopamine beta-monooxygenase) is a 73-77kD member of the copper type II ascorbate-dependent monooxygenase family.
It is both soluble (73kD) and membrane-bound (77kD) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine.
Human DbetaH is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells.
It is 603aa in length and contains a 25aa signal sequence followed by three domains.
The first is an N-terminal 120aa DOMON domain (dopamine beta-monooxygenase N-terminal) that may either bind DbetaH to the cell membrane, or participate in tetramerization.
This is followed by two 150aa Cu + -type II ascorbate-dependent monooxygenase domains (aa182-330 and 352-512).
DbetaH may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl-concentrations.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Sufficient for 10 mini-blots
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Specific for the ~75kD DβH protein in Western blots of human adrenal medulla. Species Cross Reactivity: Monkey, mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to the N-terminus region of human Dopamine beta-Hydroxylase, conjugated to (KLH).
