AlphaB Crystallin (CRYAB, also known as Crystallin alpha-B chain and HspB5) is a 22-23kD member of the HSP20 family of proteins.
It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alphaA to generate a transparent 350-1000kD alpha-Crystallin protein complex.
Human CRYAB is 175aa in length.
There is a alpha-Crystallin Hsp domain over aa66-149.
Multiple posttranslational modifications may exist.
The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved.
There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92.
There is an alternate start site at Met68 that may be accompanied by a 47aa substitution for aa109-175.
Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 0.02% sodium azide.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 100ul sterile PBS, 0.02% sodium azide.
Specificity：Recognizes human Crystallin AB (CRYAB) at ~23kD. Does not crossreact with recombinant human CRYAA in Western Blots. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli-derived recombinant human Crystallin AB (aa1-175, Accession #P02511)
